This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pNvT-MHC::mCh
catalog :
67943
citations: 3
Reference
Sebé Pedrós A, Saudemont B, Chomsky E, Plessier F, Mailhé M, Renno J, et al. Cnidarian Cell Type Diversity and Regulation Revealed by Whole-Organism Single-Cell RNA-Seq. Cell. 2018;173:1520-1534.e20 pubmed publisher
Renfer E, Technau U. Meganuclease-assisted generation of stable transgenics in the sea anemone Nematostella vectensis. Nat Protoc. 2017;12:1844-1854 pubmed publisher
Renfer E, Amon Hassenzahl A, Steinmetz P, Technau U. A muscle-specific transgenic reporter line of the sea anemone, Nematostella vectensis. Proc Natl Acad Sci U S A. 2010;107:104-8 pubmed publisher
product information
Catalog Number :
67943
Product Name :
pNvT-MHC::mCh
article :
doi10.1073/pnas.0909148107
id14696
pubmed_id20018670
bacterial resistance :
Ampicillin
cloning :
backbonepCRII-TOPO
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
transgenic reporter in the sea anemone, Nematostella vectensis
growth strain :
Transgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceI
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterMyosin Heavy Chain1 (MyHC1)
sequencing_primer_3mCherry-R
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namemCherry
shRNA_sequence
size
species
100
Synthetic
tags
resistance markers :
2478
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA