This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28-At_PRORP1-His
catalog :
67869
citations: 1
Reference
Pavlova L, Gossringer M, Weber C, Buzet A, Rossmanith W, Hartmann R. tRNA processing by protein-only versus RNA-based RNase P: kinetic analysis reveals mechanistic differences. Chembiochem. 2012;13:2270-6 pubmed publisher
product information
Catalog Number :
67869
Product Name :
pET28-At_PRORP1-His
article :
doi10.1002/cbic.201200434
id14667
pubmed_id22976545
bacterial resistance :
Kanamycin
cloning :
backbonepET28-b(+)
backbone_mutation
backbone_originNovagen
backbone_size5369
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
The mitochondrial targeting sequence MLRLTCFTPSFSRACCPLFAMMLKVPSVHLHHPRF of native precursor PRORP1 was replaced with the dipeptide MG
growth strain :
bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag
origin :
37
pi :
alt_names
At2g32230
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7 Terminal
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT2G32230, F22D22.2, F22D22_2, proteinaceous RNase P 1
genePRORP1
id817782
genbank_ids
817782
mutationResidues 36-572 (see comments)
namePRORP1
shRNA_sequence
size1611
species
3702
Arabidopsis thaliana
tags
locationC terminal on backbone
tagHis
resistance markers :
2468
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA