pSG5-FLAG-mEKLF Zinc Fingers | Addgene 67837 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSG5-FLAG-mEKLF Zinc Fingers

catalog :

67837

citations: 1

Reference
Chen X, Bieker J. Unanticipated repression function linked to erythroid Krüppel-like factor. Mol Cell Biol. 2001;21:3118-25 pubmed

product information

Catalog Number :

67837

Product Name :

pSG5-FLAG-mEKLF Zinc Fingers

article :

doi	10.1128/MCB.21.9.3118-3125.2001
id	15619
pubmed_id	11287616

bacterial resistance :

Ampicillin

cloning :

backbone

pSG5

backbone_mutation

backbone_origin

Stratagene

backbone_size

4076

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Mutations G299S, T305S have no functional consequences.

growth strain :

Expression of murine EKLF Zinc Fingers (aa 287-end) fused to Flag and driven by SV40 promoter

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	StuI
promoter	SV40
sequencing_primer_3	SV40 poly A Reverse
sequencing_primer_5	SV40pro-F2 (CCCCATGGCTGACTAATTTTT)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Eklf, Nan
gene	Klf1
id	16596

genbank_ids

mutation

Zinc Fingers are amino acids 287-end; this numbering is based on amino acid 19 as the initiator Methionine; See depositor comments below on mutations G299S, T305S

name

EKLF

shRNA_sequence

size

species

10090

Mus musculus