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product summary
company name :
Addgene
product type :
cDNA
product name :
pET14b-mEKLF
catalog :
67834
citations: 1
Reference |
---|
Armstrong J, Bieker J, Emerson B. A SWI/SNF-related chromatin remodeling complex, E-RC1, is required for tissue-specific transcriptional regulation by EKLF in vitro. Cell. 1998;95:93-104 pubmed
|
product information
Catalog Number :
67834
Product Name :
pET14b-mEKLF
article :
doi | S0092-8674(00)81785-7 [pii] |
id | 14646 |
pubmed_id | 9778250 |
bacterial resistance :
Ampicillin
cloning :
backbone | pET14b | |
backbone_mutation | ||
backbone_origin | Novagen (EMD Millipore) | |
backbone_size | 4671 | |
promoter | ||
sequencing_primer_3 | ||
sequencing_primer_5 | ||
vector_types |
|
growth notes :
The Nde1 site contains the initiator methionine of murine EKLF (entire EKLF open reading frame is defined as 71-1215 and described in PMID 7682653). Mutations A236T, G299S, and T305S have no functional consequences.
growth strain :
Encodes murine EKLF (nucleotides 71-1215) for in vitro chromatin/transcription reconstitution
growth temp :
DH5alpha is used for growth and propagation, but protein expression requires strain BL21 DE3
origin :
37
pi :
|
resistance markers :
2464 |
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments