pSG5-FLAG-mEKLF | Addgene 67833 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSG5-FLAG-mEKLF

catalog :

67833

citations: 1

Reference
Soni S, Pchelintsev N, Adams P, Bieker J. Transcription factor EKLF (KLF1) recruitment of the histone chaperone HIRA is essential for Î²-globin gene expression. Proc Natl Acad Sci U S A. 2014;111:13337-42 pubmed publisher

product information

Catalog Number :

67833

Product Name :

pSG5-FLAG-mEKLF

article :

doi	10.1073/pnas.1405422111
id	14649
pubmed_id	25197097

bacterial resistance :

Ampicillin

cloning :

backbone

pSG5

backbone_mutation

backbone_origin

Stratagene

backbone_size

4076

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Entire EKLF open reading frame is defined as nucleotides 71-1215 and described in PMID 7682653. Mutations G299S and T305S have no functional consequences.

growth strain :

Expression of FL-EKLF driven by SV40 promoter

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	StuI
promoter	SV40
sequencing_primer_3	SV40 poly A Reverse
sequencing_primer_5	SV40pro-F2 (CCCCATGGCTGACTAATTTTT)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Eklf, Nan
gene	Klf1
id	16596

genbank_ids

mutation

Full-length mEKLF is aa 20-376; amino acid 19 is the initiator Methionine; See depositor comments below on mutations G299S, T305S

name

EKLF

shRNA_sequence

size

species

10090

Mus musculus