This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pUASt- Crb intraGFP
catalog :
67525
product information
Catalog Number :
67525
Product Name :
pUASt- Crb intraGFP
article :
doi
id14566
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepUAST
backbone_mutation
backbone_origin
backbone_size8904
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
Other
Drosophila
growth notes :
The plasmid contains part of the 5' cDNA of Crb and the 3' part that brings the transmembrane and the intracellular domains of the protein together. The BglII restriction site links the 5' and 3' regions of the cDNA, meaning that the majority of the extracellular part of the Crb protein is missing. The fragment also contains the 5' and 3' UTR regions. BglII restriction site was used to clone a GFP fragment. GFP is inserted between aa 96 and 2122 of crb. Crb contains K89E mutation which does not affect plasmid function.
growth strain :
Expression of transmembrane and the intracellular domains of Crb with GFP.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5EcoRI
promoterhsp70
sequencing_primer_3SV40pA-R
sequencing_primer_5hsp70-F (GAGCGCCGGAGTATAAATAGAG)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG6383, 0509/20, 1384/04, CG6383, CRB, CT19912, Crb, Crbs, Crumbs, DmCrb, Dmel CG6383, crumb, far, l(3)07207, l(3)S050920, l(3)S058104, l(3)j1B5
genecrb
id42896
genbank_ids
mutationaa97-2103 deleted (extracellular domain)
namecrumbs
shRNA_sequence
size1379
species
7227
Drosophila melanogaster
tags
location
tagGFP
plasmid copy :
Untagged pUASt- Crb intra from Elisabeth Knust lab
resistance markers :
2445
2414
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA