This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PM-FRB-CFP
catalog :
67517
citations: 4
Reference
Kluss J, Bonet Ponce L, Lewis P, Cookson M. Directing LRRK2 to membranes of the endolysosomal pathway triggers RAB phosphorylation and JIP4 recruitment. Neurobiol Dis. 2022;170:105769 pubmed publisher
Nguyen T, Koerdt S, Gerke V. Plasma membrane phosphatidylinositol (4,5)-bisphosphate promotes Weibel-Palade body exocytosis. Life Sci Alliance. 2020;3: pubmed publisher
Lu F, Sun J, Zheng Q, Li J, Hu Y, Yu P, et al. Imaging elemental events of store-operated Ca2+ entry in invading cancer cells with plasmalemmal targeted sensors. J Cell Sci. 2019;: pubmed publisher
Varnai P, Thyagarajan B, Rohacs T, Balla T. Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells. J Cell Biol. 2006;175:377-82 pubmed
product information
Catalog Number :
67517
Product Name :
PM-FRB-CFP
article :
doi10.1083/jcb.200607116
id14560
pubmed_id17088424
bacterial resistance :
Kanamycin
cloning :
backboneCFP
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
FRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS
geneMTOR
id2475
genbank_ids
mutationFRB domain only (residues 2019 2114)
namemTOR
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagPM tethering sequence (MLCCMRRTKQVEKNDDDQKI) from human GAP43 (residues 1 20)
locationC terminal on insert
tagHA
locationC terminal on insert
tagCFP
resistance markers :
1857
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA