This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV3-SMAC-HA-eGFP
catalog :
67489
citations: 1
Reference
Wong Y, Ysselstein D, Krainc D. Mitochondria-lysosome contacts regulate mitochondrial fission via RAB7 GTP hydrolysis. Nature. 2018;554:382-386 pubmed publisher
product information
Catalog Number :
67489
Product Name :
pCMV3-SMAC-HA-eGFP
article :
doi
id14551
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepCMV(delta3)
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the IMS using the leader sequence from SMAC/Diablo: Sabharwal, et al (2013) Biochem J 456: 337-46, and (3) Ozawa, et al (2007) ACS Chemical Biology 2: 176-186.
growth strain :
Targets HA-eGFP to mitochondrial intermembrane space (IMS)
origin :
37
pi :
alt_names
pRK111
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5KpnI
promoterattenuated CMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDFNA64, SMAC
geneDIABLO
id56616
genbank_ids
mutationaa1-59
nameSMAC
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHA-eGFP
resistance markers :
2363
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA