This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONR221-ARF4-no stop
catalog :
67311
product information
Catalog Number :
67311
Product Name :
pDONR221-ARF4-no stop
article :
doi
id14551
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepDONR221
backbone_mutation
backbone_originLife Technologies
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Other
Gateway Entry vector
growth notes :
Please note that pDSHA-ARF4-Myc and pDEST47-ARF4-GFP (Addgene plasmid #s 67350 & 67392) were created from this plasmid and also contain the V53A, L123P and M134I mutations in ARF4. These mutations were unintentionally introduced during PCR.
growth strain :
hArf4 without stop codon in Gateway Entry vector
origin :
37
pi :
alt_names
pRK3s-221
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13-rev
sequencing_primer_5M13-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesARF2
geneARF4
id378
genbank_ids
mutationV53A, L123P and M134I mutations were unintended PCR-generated artifacts
nameARF4
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
2363
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA