This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1_AktPH-mCherry
catalog :
67301
citations: 3
Reference
Snider C, Willet A, Brown H, Gould K. Analysis of the contribution of phosphoinositides to medial septation in fission yeast highlights the importance of PI(4,5)P2 for medial contractile ring anchoring. Mol Biol Cell. 2018;29:2148-2155 pubmed publisher
Vashi N, Andrabi S, Ghanwat S, Suar M, Kumar D. Ca2+-dependent Focal Exocytosis of Golgi-derived Vesicles Helps Phagocytic Uptake in Macrophages. J Biol Chem. 2017;292:5144-5165 pubmed publisher
Kawano F, Suzuki H, Furuya A, Sato M. Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat Commun. 2015;6:6256 pubmed publisher
product information
Catalog Number :
67301
Product Name :
pcDNA3.1_AktPH-mCherry
article :
doi10.1038/ncomms7256
id10330
pubmed_id25708714
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_originInvitrogen
backbone_size5348
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Fluorescent indicator for PI(3,4,5)P3
origin :
37
pi :
alt_names
pleckstrin homology (PH) domain derived from Akt1
cloning
clone_methodRestriction Enzyme
cloning_site_3ApaI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
geneAKT1
id207
genbank_ids
mutation
nameAktPH-mCherry
shRNA_sequence
size2042
species
9606
Homo sapiens
100
Synthetic
tags
resistance markers :
2290
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA