This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
HaloTag-beta2-adrenergic receptor
catalog :
66994
citations: 1
Reference
Frei M, Hoess P, Lampe M, Nijmeijer B, Kueblbeck M, Ellenberg J, et al. Photoactivation of silicon rhodamines via a light-induced protonation. Nat Commun. 2019;10:4580 pubmed publisher
product information
Catalog Number :
66994
Product Name :
HaloTag-beta2-adrenergic receptor
article :
doi
id14499
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backboneDerived from pHT2
backbone_mutation
backbone_origin
backbone_size3989
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Beta2-adrenergic receptor insert (without the signal sequence) can be retrieved using Ngo MIV.
growth strain :
Beta-2-adrenergic receptor tagged with HaloTag on amino terminus
origin :
37
pi :
alt_names
ADRB2
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NheI
promoterCMV
sequencing_primer_3EBV Reverse
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR
geneADRB2
id154
genbank_ids
mutationThe signal sequence from human growth hormone was added to the N-terminus of HaloTag, which was then added to the amino terminus of the beta2-adrenergic receptor.
nameHaloTag-beta2-adrenergic receptor
shRNA_sequence
size2297
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHuman Growth hormone Signal Sequence-HaloTag
plasmid copy :
Beta-2-adrenergic receptor-GFP was obtained from Gerda Breitweiser, Weis Center for Research, Geisinger Clinic, Danville, PA. HaloTag pHT2 was purchased from Promega.
resistance markers :
2434
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA