G protein alpha-s-HaloTag | Addgene 66993 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

G protein alpha-s-HaloTag

catalog :

66993

product information

Catalog Number :

66993

Product Name :

G protein alpha-s-HaloTag

article :

doi
id	14499
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNAI/Amp

backbone_mutation

backbone_origin

Invitrogen

backbone_size

4800

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

G protein alpha-s internally tagged with HaloTag and EE epitope

origin :

pi :

alt_names

Gnas

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	HindIII
promoter	CMV
sequencing_primer_3	SP6
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALEX, G-alpha-8, Gnas1, Gnpas, Nesp55, SCG6
gene	Gnas
id	24896

genbank_ids

mutation

BamHI sites in the alpha-s cDNA were removed and alpha-s residues 73-84 were replaced with Gly-Ser adding a unique BamHI site into which HaloTag was subcloned.

name

G-alpha-s-EE-HaloTag

shRNA_sequence

size

2485

species

10116

Rattus norvegicus

tags

location
tag	HaloTag flanked by SGGGGSGGGGS on each side was inserted in place of alpha-s residues 73- 84.

location
tag	internal EE epitope (residues 189-194 in alpha- s sequence are EYMPTE)

plasmid copy :

Alpha-s-EE was produced in the laboratory of Henry Bourne, University of California, San Francisco, CA. HaloTag pHT2 was purchased from Promega.

resistance markers :

2434

tags :

High Copy