pPD95.75 RSF-1 | Addgene 66863 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pPD95.75 RSF-1

catalog :

66863

citations: 1

Reference
Iwasa H, Kuroyanagi H, Maimaiti S, Ikeda M, Nakagawa K, Hata Y. Characterization of RSF-1, the Caenorhabditis elegans homolog of the Ras-association domain family protein 1. Exp Cell Res. 2013;319:1-11 pubmed publisher

product information

Catalog Number :

66863

Product Name :

pPD95.75 RSF-1

article :

doi	10.1016/j.yexcr.2012.10.008
id	14446
pubmed_id	23103556

bacterial resistance :

Ampicillin

cloning :

backbone

PD95.75

backbone_mutation

backbone_origin

Addgene

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Worm Expression

growth notes :

I performed PCR on C.elegans genome to isolate the genome of RSF-1 which contain 5' UTR and the coding exons and introns. I subcloned the product into PstI/SmaI sites of PD95.75, so that GFP is fused to the last exon.

growth strain :

Generate transgenic C. elegans expressing GFP-fused RSF-1

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SmaI
cloning_site_5	PstI
promoter
sequencing_primer_3	GFP-R primer
sequencing_primer_5	M13 reverse
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CELE_T24F1.3
gene	rsf-1
id	174728

genbank_ids

mutation

name

RSF-1

shRNA_sequence

size

species

6239

Caenorhabditis elegans