pClneoEGFPC-let60 K16N | Addgene 66861 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pClneoEGFPC-let60 K16N

catalog :

66861

citations: 1

Reference
Iwasa H, Kuroyanagi H, Maimaiti S, Ikeda M, Nakagawa K, Hata Y. Characterization of RSF-1, the Caenorhabditis elegans homolog of the Ras-association domain family protein 1. Exp Cell Res. 2013;319:1-11 pubmed publisher

product information

Catalog Number :

66861

Product Name :

pClneoEGFPC-let60 K16N

article :

doi	10.1016/j.yexcr.2012.10.008
id	14446
pubmed_id	23103556

bacterial resistance :

Ampicillin

cloning :

backbone

pClneo

backbone_mutation

backbone_origin

Promega

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Express GFP-tagged dominat negative let60, in which Lysine 16 is mutated to asparagine, in mammalian cells

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	Xhoi
cloning_site_5	EcoRI
promoter	CMV
sequencing_primer_3	T3 promoter
sequencing_primer_5	EGFP-C
site_3_destroyed	1
site_5_destroyed

entrez_gene

aliases	CELE_ZK792.6
gene	let-60
id	178104

genbank_ids

mutation

Lysine 16 is mutated to asparagine

name

Let-60

shRNA_sequence

size

species

6239

Caenorhabditis elegans