pClneoFH-let60 G12V | Addgene 66860 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pClneoFH-let60 G12V

catalog :

66860

citations: 2

Reference
Victor M, Richner M, Olsen H, Lee S, Monteys A, Ma C, et al. Striatal neurons directly converted from Huntington's disease patient fibroblasts recapitulate age-associated disease phenotypes. Nat Neurosci. 2018;21:341-352 pubmed publisher
Iwasa H, Kuroyanagi H, Maimaiti S, Ikeda M, Nakagawa K, Hata Y. Characterization of RSF-1, the Caenorhabditis elegans homolog of the Ras-association domain family protein 1. Exp Cell Res. 2013;319:1-11 pubmed publisher

product information

Catalog Number :

66860

Product Name :

pClneoFH-let60 G12V

article :

doi	10.1016/j.yexcr.2012.10.008
id	14446
pubmed_id	23103556

bacterial resistance :

Ampicillin

cloning :

backbone

pClneo

backbone_mutation

backbone_origin

Promega

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Express FLAG-His6-tagged dominant active let60, in which Glycine 12 is mutated to Valine, in mammalian cells

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	EcoRI
promoter	CMV
sequencing_primer_3	T3 promoter
sequencing_primer_5	CMV promoter
site_3_destroyed	1
site_5_destroyed

entrez_gene

aliases	CELE_ZK792.6
gene	let-60
id	178104

genbank_ids

mutation

Glycine 12 is mutated to valine

name

Let-60

shRNA_sequence

size

species

6239

Caenorhabditis elegans