pPD95.75-cYAP-1 S104A | Addgene 66858 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pPD95.75-cYAP-1 S104A

catalog :

66858

citations: 1

Reference
Iwasa H, Maimaiti S, Kuroyanagi H, Kawano S, Inami K, Timalsina S, et al. Yes-associated protein homolog, YAP-1, is involved in the thermotolerance and aging in the nematode Caenorhabditis elegans. Exp Cell Res. 2013;319:931-45 pubmed publisher

product information

Catalog Number :

66858

Product Name :

pPD95.75-cYAP-1 S104A

article :

doi	10.1016/j.yexcr.2013.01.020
id	14445
pubmed_id	23396260

bacterial resistance :

Ampicillin

cloning :

backbone

PD95.75

backbone_mutation

backbone_origin

Addgene

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Worm Expression

growth notes :

I performed PCR on C.elegans genome to isolate the genome of YAP-1 which contain 5' UTR and the coding exons and introns. I subcloned the product into PstI/SmaI sites of PD95.75, so that GFP is fused to the last exon. Serine 104 was mutated to alanine.

growth strain :

Generate transgenic C.elegans with cYAP1- fused to GFP, in which Serine 104 is mutated to alanine

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SmaI
cloning_site_5	PstI
promoter
sequencing_primer_3	GFP-R primer
sequencing_primer_5	M13 reverse
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CELE_F13E6.4
gene	yap-1
id	181267

genbank_ids

mutation

Serine104 is mutated to alanine

name

YAP-1

shRNA_sequence

size

species

6239

Caenorhabditis elegans