This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET30Xa-SBL1(160E)
catalog :
66846
citations: 1
Reference
Coffa G, Imber A, Maguire B, Laxmikanthan G, Schneider C, Gaffney B, et al. On the relationships of substrate orientation, hydrogen abstraction, and product stereochemistry in single and double dioxygenations by soybean lipoxygenase-1 and its Ala542Gly mutant. J Biol Chem. 2005;280:38756-66 pubmed
product information
Catalog Number :
66846
Product Name :
pET30Xa-SBL1(160E)
article :
doi10.1074/jbc.M504870200
id14438
pubmed_id16157595
bacterial resistance :
Kanamycin
cloning :
backbonepET30Xa-LIC
backbone_mutation11 bp added after coding region: TAAACTAGTCATGGGGC
backbone_originNovagen
backbone_size5444
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Cloned ~1996 from green pods of plants grown using organic soybeans from Glacial Ridge Foods. Brendan C. Maguire, Dissertation, Johns Hopkins University, 1999, UMI Microform: 9927119.
growth strain :
Expresses soybean lipoxygenase-1 S160E mutant with N-term Histag
growth temp :
37C before induction, 30 deg for 14-16+ hrs after induction
origin :
37
pi :
alt_names
SBL1
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterT7lac
sequencing_primer_3TTGCCGACTCTCATTAGCC
sequencing_primer_5TCATCATTCTTCTGGTCTGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGLYMA_08G189200, LOX
geneLOC547694
id547694
aliases
geneNEWENTRY
id3760024
genbank_ids
GB_AAA33986.1
mutationS160E
namesoybean lipoxygenase-1
shRNA_sequence
size2517
species
32644
Other
Glycine max
tags
locationN terminal on backbone
tagHis-tag
resistance markers :
2421
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA