This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSLIK-IDH2-R172K-FLAG
catalog :
66807
citations: 5
Reference
Sabatier M, Boet E, Zaghdoudi S, Guiraud N, Hucteau A, Polley N, et al. Activation of Vitamin D Receptor Pathway Enhances Differentiating Capacity in Acute Myeloid Leukemia with Isocitrate Dehydrogenase Mutations. Cancers (Basel). 2021;13: pubmed publisher
Parker S, Encarnación Rosado J, Hollinshead K, Hollinshead D, Ash L, Rossi J, et al. Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters. Nat Commun. 2021;12:4905 pubmed publisher
Lim E, Parker S, Metallo C. Deuterium Tracing to Interrogate Compartment-Specific NAD(P)H Metabolism in Cultured Mammalian Cells. Methods Mol Biol. 2020;2088:51-71 pubmed publisher
McBrayer S, Mayers J, DiNatale G, Shi D, Khanal J, Chakraborty A, et al. Transaminase Inhibition by 2-Hydroxyglutarate Impairs Glutamate Biosynthesis and Redox Homeostasis in Glioma. Cell. 2018;175:101-116.e25 pubmed publisher
Lewis C, Parker S, Fiske B, McCloskey D, Gui D, Green C, et al. Tracing compartmentalized NADPH metabolism in the cytosol and mitochondria of mammalian cells. Mol Cell. 2014;55:253-63 pubmed publisher
product information
Catalog Number :
66807
Product Name :
pSLIK-IDH2-R172K-FLAG
article :
doi10.1016/j.molcel.2014.05.008
id14420
pubmed_id24882210
bacterial resistance :
Ampicillin
cloning :
backbonepSLIK-hygro
backbone_mutation
backbone_originIan Frasier, Addgene Plasmid #25737
backbone_size13918
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Insert gene obtained from Patrick Ward and Craig Thompson in the form of pLPCX-IDH2-R172K Addgene has not confirmed the R132H mutation within this plasmid. The depositor states that this plasmid behaves correctly based on on 2HG production (i.e. the metabolic activity of the enzyme).
growth strain :
Tet-inducible expression of mutant IDH2 in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3huB-Cpro-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesD2HGA2, ICD-M, IDH, IDH-2, IDHM, IDP, IDPM, mNADP-IDH
geneIDH2
id3418
genbank_ids
NM_002168.3
mutationR172K
nameIDH2
shRNA_sequence
size1471
species
9606
Homo sapiens
tags
locationC terminal on insert
tag3xFLAG
resistance markers :
2416
tags :
Unknown
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA