This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSLIK-IDH2-FLAG
catalog :
66806
citations: 2
Reference
Sabatier M, Boet E, Zaghdoudi S, Guiraud N, Hucteau A, Polley N, et al. Activation of Vitamin D Receptor Pathway Enhances Differentiating Capacity in Acute Myeloid Leukemia with Isocitrate Dehydrogenase Mutations. Cancers (Basel). 2021;13: pubmed publisher
Lewis C, Parker S, Fiske B, McCloskey D, Gui D, Green C, et al. Tracing compartmentalized NADPH metabolism in the cytosol and mitochondria of mammalian cells. Mol Cell. 2014;55:253-63 pubmed publisher
product information
Catalog Number :
66806
Product Name :
pSLIK-IDH2-FLAG
article :
doi10.1016/j.molcel.2014.05.008
id14420
pubmed_id24882210
bacterial resistance :
Ampicillin
cloning :
backbonepSLIK-hygro
backbone_mutation
backbone_originIan Frasier, Addgene Plasmid #25737
backbone_size13918
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Addgene has not confirmed the n-terminus of IDH2. Depositor states that this plasmid functions correctly based on 2HG production (i.e. the metabolic activity of the enzyme).
growth strain :
Tet-inducible expression of IDH2 in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3huB-Cpro-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesD2HGA2, ICD-M, IDH, IDH-2, IDHM, IDP, IDPM, mNADP-IDH
geneIDH2
id3418
genbank_ids
NM_002168.3
mutation
nameIDH2
shRNA_sequence
size1471
species
9606
Homo sapiens
tags
locationC terminal on insert
tag3xFLAG
resistance markers :
2416
tags :
Unknown
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA