This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSLIK-IDH1-R132H-FLAG
catalog :
66803
citations: 6
Reference
Mehjardi N, Kessler J, Sanin A, Picard D, Westhoff P, Nickel A, et al. The development of a hiPSC-based platform to identify tissue-dependencies of IDH1 R132H. Cell Death Discov. 2023;9:452 pubmed publisher
Yang F, Wang C, Zhu J, Gai Q, Mao M, He J, et al. Inhibitory effects of temozolomide on glioma cells is sensitized by RSL3-induced ferroptosis but negatively correlated with expression of ferritin heavy chain 1 and ferritin light chain. Lab Invest. 2022;102:741-752 pubmed publisher
Parker S, Encarnación Rosado J, Hollinshead K, Hollinshead D, Ash L, Rossi J, et al. Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters. Nat Commun. 2021;12:4905 pubmed publisher
Garbarino J, Eckroate J, Sundaram R, Jensen R, Bindra R. Loss of ATRX confers DNA repair defects and PARP inhibitor sensitivity. Transl Oncol. 2021;14:101147 pubmed publisher
Lim E, Parker S, Metallo C. Deuterium Tracing to Interrogate Compartment-Specific NAD(P)H Metabolism in Cultured Mammalian Cells. Methods Mol Biol. 2020;2088:51-71 pubmed publisher
Lewis C, Parker S, Fiske B, McCloskey D, Gui D, Green C, et al. Tracing compartmentalized NADPH metabolism in the cytosol and mitochondria of mammalian cells. Mol Cell. 2014;55:253-63 pubmed publisher
product information
Catalog Number :
66803
Product Name :
pSLIK-IDH1-R132H-FLAG
article :
doi10.1016/j.molcel.2014.05.008
id14420
pubmed_id24882210
bacterial resistance :
Ampicillin
cloning :
backbonepSLIK-hygro
backbone_mutation
backbone_originIan Frasier, Addgene Plasmid #25737
backbone_size13918
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
The depositor states that this plasmid behaves correctly based on on 2HG production (i.e. the metabolic activity of the enzyme).
growth strain :
Tet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vector
origin :
37
pi :
alt_names
isocitrate dehydrogenase
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3huB-Cpro-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD
geneIDH1
id3417
genbank_ids
NM_005896.3
mutationR132H
nameIDH1
shRNA_sequence
size1357
species
9606
Homo sapiens
tags
locationC terminal on insert
tag3xFLAG
plasmid copy :
Insert gene obtained from Patrick Ward and Craig Thompson in the form of pLPCX-IDH1-R132H
resistance markers :
2416
tags :
Unknown
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA