This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CmCitrine Lck w/ NosUTRs
catalog :
66785
citations: 1
Reference
Campanale J, Gokirmak T, Espinoza J, Oulhen N, Wessel G, Hamdoun A. Migration of sea urchin primordial germ cells. Dev Dyn. 2014;243:917-27 pubmed publisher
product information
Catalog Number :
66785
Product Name :
CmCitrine Lck w/ NosUTRs
article :
doi10.1002/dvdy.24133
id14409
pubmed_id24677545
bacterial resistance :
Ampicillin
cloning :
backbonepGEM-T
backbone_mutation
backbone_originPromega
backbone_size3000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
sea urchin, zebrafish, xenopus
growth notes :
The membrane anchoring of mCitrine in sea urchin germ cells uses the sea urchin (S. purpuratus) 5' and 3' UTRs Nanos2 UTRs flanking this insert.
growth strain :
expression of fluorescent membranes in sea urchin primordial germ cells
origin :
37
pi :
alt_names
LCK proto-oncogene, Src family tyrosine kinase
cloning
clone_methodRestriction Enzyme
cloning_site_3NdeI
cloning_site_5ApaI
promoterT7
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesIMD22, LSK, YT16, p56lck, pp58lck
geneLCK
id3932
genbank_ids
mutationTandem repeat of LCK the N-terminal membrane targeting sequence: MGCIKSKRKDNLNDDEAAMGCIKSKRKDNLNDDEAPVAT
nameLck
shRNA_sequence
size2144
species
9606
Homo sapiens
tags
locationN terminal on insert
tag5' UTR from sea urchin Nanos
locationC terminal on insert
tagmCitrine
locationC terminal on insert
tag3' UTR from sea urchin Nanos
plasmid copy :
Lck was taken from Addgene plasmid #53749
resistance markers :
1186
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA