pLVX-Puro-mRuby-WDR34 | Addgene 66599 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLVX-Puro-mRuby-WDR34

catalog :

66599

citations: 1

Reference
Asante D, Stevenson N, Stephens D. Subunit composition of the human cytoplasmic dynein-2 complex. J Cell Sci. 2014;127:4774-87 pubmed publisher

product information

Catalog Number :

66599

Product Name :

pLVX-Puro-mRuby-WDR34

article :

doi	10.1242/jcs.159038
id	9965
pubmed_id	25205765

bacterial resistance :

Ampicillin

cloning :

backbone

pLVX-Puro

backbone_mutation

backbone_origin

Clontech

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

The cDNA sequence encoding mRuby (Kredel et al., 2009) underwent codon usage adaptation before gene synthesis (MWG Eurofins) and cloning into pBluescript II SK(+). mRuby was excised with SalI-EcoRI for insertion into pLVX-Puro (Clontech) to generate pLVX-Puro mRuby. A cDNA encoding human Sec23A was amplified by PCR, gel purified and subcloned via pGEM-T vector (excised with ApaI-XbaI) and cloned into pLVX-Puro mRuby.

growth strain :

Expresses WDR34 as a fusion with monomeric mRuby

origin :

pi :

alt_names

WD34

FAP133

cloning

entrez_gene

aliases	DIC5, FAP133, SRTD11, bA216B9.3
gene	WDR34
id	89891

genbank_ids

NM_052844.3

mutation

name

WDR34

shRNA_sequence

size

species

9606

Homo sapiens