This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GPR148-Tango
catalog :
66323
citations: 1
Reference
Kroeze W, Sassano M, Huang X, Lansu K, McCorvy J, Giguère P, et al. PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome. Nat Struct Mol Biol. 2015;22:362-9 pubmed publisher
product information
Catalog Number :
66323
Product Name :
GPR148-Tango
article :
doi10.1038/nsmb.3014
id10377
pubmed_id25895059
bacterial resistance :
Ampicillin
cloning :
backboneempty Tango
backbone_mutation
backbone_origin
backbone_size6632
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note: Most of the PRESTO-Tango vectors do not contain the XhoI cut site downstream of the tTA sequence and instead carry an XbaI site. If you need to perform an XhoI digest we recommend sequencing with a forward primer at the C-terminus of tTA to determine which of the two sites is present. Suggested primer: 5-gagctccacttagacggcgagg-3. Original backbone was pcDNA3.1(+). These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
growth strain :
Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NotI
promoterCMV
sequencing_primer_3Tet-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBTR, PGR6
geneGPR148
id344561
genbank_ids
mutation
nameGPR148
shRNA_sequence
size1041
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG
resistance markers :
1493
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA