GLP1R-Tango | Addgene 66295 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

GLP1R-Tango

catalog :

66295

citations: 9

Reference
Park J, Bak M, Min K, Kim H, Cho J, Tae G, et al. Effect of C-terminus Conjugation via Different Conjugation Chemistries on In Vivo Activity of Albumin-Conjugated Recombinant GLP-1. Pharmaceutics. 2021;13: pubmed publisher
Arcones A, Vila Bedmar R, Mirasierra M, Cruces Sande M, Vallejo M, Jones B, et al. GRK2 regulates GLP-1R-mediated early phase insulin secretion in vivo. BMC Biol. 2021;19:40 pubmed publisher
Pickford P, Lucey M, Fang Z, Bitsi S, de la Serna J, Broichhagen J, et al. Signalling, trafficking and glucoregulatory properties of glucagon-like peptide-1 receptor agonists exendin-4 and lixisenatide. Br J Pharmacol. 2020;177:3905-3923 pubmed publisher
Bak M, Park J, Min K, Cho J, Seong J, Hahn Y, et al. Recombinant Peptide Production Platform Coupled with Site-Specific Albumin Conjugation Enables a Convenient Production of Long-Acting Therapeutic Peptide. Pharmaceutics. 2020;12: pubmed publisher
Fang Z, Chen S, Pickford P, Broichhagen J, Hodson D, Correa I, et al. The Influence of Peptide Context on Signaling and Trafficking of Glucagon-like Peptide-1 Receptor Biased Agonists. ACS Pharmacol Transl Sci. 2020;3:345-360 pubmed publisher
Song X, Yu Y, Shen C, Wang Y, Wang N. Dimerization/oligomerization of the extracellular domain of the GLP-1 receptor and the negative cooperativity in its ligand binding revealed by the improved NanoBiT. FASEB J. 2020;34:4348-4368 pubmed publisher
Buenaventura T, Bitsi S, Laughlin W, Burgoyne T, Lyu Z, Oqua A, et al. Agonist-induced membrane nanodomain clustering drives GLP-1 receptor responses in pancreatic beta cells. PLoS Biol. 2019;17:e3000097 pubmed publisher
Traub S, Meier D, Schulze F, Dror E, Nordmann T, Goetz N, et al. Pancreatic α Cell-Derived Glucagon-Related Peptides Are Required for β Cell Adaptation and Glucose Homeostasis. Cell Rep. 2017;18:3192-3203 pubmed publisher
Kroeze W, Sassano M, Huang X, Lansu K, McCorvy J, GiguÃ¨re P, et al. PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome. Nat Struct Mol Biol. 2015;22:362-9 pubmed publisher

product information

Catalog Number :

66295

Product Name :

GLP1R-Tango

article :

doi	10.1038/nsmb.3014
id	10377
pubmed_id	25895059

bacterial resistance :

Ampicillin

cloning :

backbone

empty Tango

backbone_mutation

backbone_origin

backbone_size

6632

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Please note: Most of the PRESTO-Tango vectors do not contain the XhoI cut site downstream of the tTA sequence and instead carry an XbaI site. If you need to perform an XhoI digest we recommend sequencing with a forward primer at the C-terminus of tTA to determine which of the two sites is present. Suggested primer: 5-gagctccacttagacggcgagg-3. Original backbone was pcDNA3.1(+). These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.

growth strain :

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NotI
promoter	CMV
sequencing_primer_3	Tet-R
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	GLP-1, GLP-1-R, GLP-1R
gene	GLP1R
id	2740

genbank_ids

mutation

name

GLP1R

shRNA_sequence

size

1389

species

9606

Homo sapiens