This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
APEX2 - Actin in pEGFP
catalog :
66172
citations: 5
Reference
Oh N, Tarte N. Subcellular distribution of the rAAV genome depends on genome structure. Sci Rep. 2023;13:17325 pubmed publisher
Xu H, Bensalel J, Raju S, Capobianco E, Lu M, Wei J. Characterization of huntingtin interactomes and their dynamic responses in living cells by proximity proteomics. J Neurochem. 2022;: pubmed publisher
Kong H, Zhang J, Li J, Wang J, Shin H, Tai R, et al. Genetically encoded X-ray cellular imaging for nanoscale protein localization. Natl Sci Rev. 2020;7:1218-1227 pubmed publisher
Lin W, Chakraborty A, Huang S, Wang P, Hsieh Y, Chien K, et al. Histidine-Dependent Protein Methylation Is Required for Compartmentalization of CTP Synthase. Cell Rep. 2018;24:2733-2745.e7 pubmed publisher
Lam S, Martell J, Kamer K, Deerinck T, Ellisman M, Mootha V, et al. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12:51-4 pubmed publisher
product information
Catalog Number :
66172
Product Name :
APEX2 - Actin in pEGFP
article :
doi10.1038/nmeth.3179
id9423
pubmed_id25419960
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP
backbone_mutation
backbone_originClonetech
backbone_size3954
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
APEX2 fused to the N-terminus of human beta actin
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NheI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMVf (CGCAAATGGGCGGTAGGCGTG)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8
geneACTB
id60
genbank_ids
mutationAPEX2 contains 4 mutations relative to wt soybean APX: K14D, W41F, E112K, A134P
nameAPEX2 - human beta Actin
shRNA_sequence
size1953
species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on insert
tagFlag epitope tag (DYKDDDDK)
resistance markers :
526
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA