This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Vimentin - APEX2 in pECFP
catalog :
66170
citations: 2
Reference
Bram Y, Duan X, Nilsson Payant B, Chandar V, Wu H, Shore D, et al. Dual-Reporter System for Real-Time Monitoring of SARS-CoV-2 Main Protease Activity in Live Cells Enables Identification of an Allosteric Inhibition Path. ACS Bio Med Chem Au. 2022;2:627-641 pubmed publisher
Lam S, Martell J, Kamer K, Deerinck T, Ellisman M, Mootha V, et al. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12:51-4 pubmed publisher
product information
Catalog Number :
66170
Product Name :
Vimentin - APEX2 in pECFP
article :
doi10.1038/nmeth.3179
id9423
pubmed_id25419960
bacterial resistance :
Kanamycin
cloning :
backbonepECFP
backbone_mutation
backbone_originClonetech
backbone_size3970
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
APEX2 fused to the C-terminus human vimentin
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NheI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMVf (CGCAAATGGGCGGTAGGCGTG)
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneVIM
id7431
genbank_ids
mutationAPEX2 contains 4 mutations relative to wt soybean APX: K14D, W41F, E112K, A134P
namehuman Vimentin - APEX2
shRNA_sequence
size2193
species
9606
Homo sapiens
100
Synthetic
tags
locationC terminal on insert
tagFlag epitope tag (DYKDDDDK)
resistance markers :
526
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA