This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET11+PCNA3-CYP119(T214V)
catalog :
66137
citations: 1
Reference
Suzuki R, Hirakawa H, Nagamune T. Electron donation to an archaeal cytochrome P450 is enhanced by PCNA-mediated selective complex formation with foreign redox proteins. Biotechnol J. 2014;9:1573-81 pubmed publisher
product information
Catalog Number :
66137
Product Name :
pET11+PCNA3-CYP119(T214V)
article :
doi10.1002/biot.201400007
id10356
pubmed_id24924478
bacterial resistance :
Ampicillin
cloning :
backbonepET-11a
backbone_mutation
backbone_originNovagen
backbone_size5639
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
We cloned the CYP119 gene from the cell suspension of S. solfataricus P1 strain (ATCC 35091).
growth strain :
Expresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3
growth temp :
Supplement with 1% glucose is recommended to suppress leaky expression.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promoterT7 promoter
sequencing_primer_3T7 terminator
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSaci_2081
genecyp119
id3472594
genbank_ids
mutationT214V
nameCYP119
shRNA_sequence
size1928
species
32644
Other
tags
locationN terminal on insert
tagSulfolobus solfataricus PCNA3
resistance markers :
2340
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA