This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
G protein alpha-s-mCerulean
catalog :
66084
citations: 1
Reference
Hynes T, Mervine S, Yost E, Sabo J, Berlot C. Live cell imaging of Gs and the beta2-adrenergic receptor demonstrates that both alphas and beta1gamma7 internalize upon stimulation and exhibit similar trafficking patterns that differ from that of the beta2-adrenergic receptor. J Biol Chem. 2004;279:44101-12 pubmed
product information
Catalog Number :
66084
Product Name :
G protein alpha-s-mCerulean
article :
doi10.1074/jbc.M405151200
id8567
pubmed_id15297467
bacterial resistance :
Ampicillin
cloning :
backbonepcDNAI/Amp
backbone_mutation
backbone_originInvitrogen
backbone_size4800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Reference for alpha-s-EE: Wilson, P. T., and Bourne, H. R. (1995) Fatty acylation of z. Effects of palmitoylation and myristoylation on z signaling. J. Biol. Chem. 270, 9667-9675 Reference for mCerulean: Rizzo MA, Springer GH, Granada B, Piston DW. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol. 2004;22(4):445-9. PubMed PMID: 14990965.
growth strain :
G protein alpha-s internally tagged with mCerulean and EE epitope
origin :
37
pi :
alt_names
Gnas
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5HindIII
promoterCMV
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALEX, G-alpha-8, Gnas1, Gnpas, Nesp55, SCG6
geneGnas
id24896
genbank_ids
mutationBamHI sites in the alpha-s cDNA were removed and alpha-s residues 73-84 were replaced with Gly-Ser adding a unique BamHI site into which mCerulean was subcloned.
nameG-alpha-s-EE-mCerulean
shRNA_sequence
size2303
species
10116
Rattus norvegicus
32644
Other
Aequorea victoria
tags
location
tagmCerulean flanked by SGGGS on each side was inserted in place of alpha-s residues 73-84.
location
taginternal EE epitope (residues 189-194 in alpha-s sequence are EYMPTE)
plasmid copy :
Alpha-s-EE was produced in the laboratory of Henry Bourne, University of California, San Francisco, CA. Monomeric Cerulean was obtained from David Piston, Vanderbilt University, Nashville, TN.
resistance markers :
2434
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA