pCaSpeR3/ncd-gfp #10 | Addgene 66082 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCaSpeR3/ncd-gfp #10

catalog :

66082

citations: 1

Reference
Endow S, Komma D. Spindle dynamics during meiosis in Drosophila oocytes. J Cell Biol. 1997;137:1321-36 pubmed

product information

Catalog Number :

66082

Product Name :

pCaSpeR3/ncd-gfp #10

article :

doi
id	10327
pubmed_id	9182665

bacterial resistance :

Ampicillin

cloning :

backbone

pCaSpeR3

backbone_mutation

Plasmid contains 3.1kb ncd promoter, 2.1kb ncd coding region and 0.7kb GFP

backbone_origin

backbone_size

7814

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth notes :

Other references describing Ncd-GFP expression: Endow SA & Komma DJ 1996 J Cell Sci 109, 2429-2442 - PubMed ID: 8923204 Skold HN, Komma DJ & Endow SA 2005 J Cell Sci 118, 1745-1755 - PubMed ID: 15797926

growth strain :

stable germline transformation of Drosophila to produce Ncd-GFP expression

growth temp :

Medium copy number plasmid; Preferred growth temperature 34-35C

origin :

pi :

alt_names

Ca[nd]

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BglII
promoter	ncd promoter (3.1kb upstream region of ncd start site)
sequencing_primer_3	Cas3 Rev (5'-AACTCATAATATTAATTAGACG-3')
sequencing_primer_5	pCasper-F (5'-GGGTTTTATTAACTTACAT-3')
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	Dmel_CG7831, CG7831, DmNCD, DmNcd, Dmel CG7831, KIF C1, KIFC1, NCD, Ncd, ca, ca[nd], cd, l(3)05884, ndc
gene	ncd
id	43517

genbank_ids

X52814

mutation

D699L

name

Ncd kinesin-14 motor protein

shRNA_sequence

size

5900

species

7227

Drosophila melanogaster