This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
213-petDuet1-GBD-LUMP--cdc42
catalog :
65973
citations: 1
Reference
Hoepker A, Wang A, Le Marois A, Suhling K, Yan Y, Marriott G. Genetically encoded sensors of protein hydrodynamics and molecular proximity. Proc Natl Acad Sci U S A. 2015;112:E2569-74 pubmed publisher
product information
Catalog Number :
65973
Product Name :
213-petDuet1-GBD-LUMP--cdc42
article :
doi10.1073/pnas.1424021112
id10304
pubmed_id25931526
bacterial resistance :
Ampicillin
cloning :
backbonepetDuet1
backbone_mutation
backbone_origin
backbone_size5321
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Dual expression of GBD-LUMP and cdc42
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5NcoI
promoterT7
sequencing_primer_3
sequencing_primer_5pBRrevBam
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameLUMP
shRNA_sequence
size702
species
32644
Other
Photobacterium leignathi
tags
locationN terminal on insert
tagGBD (GTPase binding domain from ACK1; residues 448 489)
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7 terminal
sequencing_primer_5none
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDC42Hs, G25K, TKS
geneCDC42
id998
genbank_ids
mutationconstitutively active Cdc42 (Q61L)
namecell division cycle 42
shRNA_sequence
size
species
9606
Homo sapiens
tags
plasmid copy :
cdc42 from Addgene.
resistance markers :
2387
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA