This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Pflp-18::NGL-1::spGFP11
catalog :
65828
citations: 1
Reference
Feinberg E, VanHoven M, Bendesky A, Wang G, Fetter R, Shen K, et al. GFP Reconstitution Across Synaptic Partners (GRASP) defines cell contacts and synapses in living nervous systems. Neuron. 2008;57:353-63 pubmed publisher
product information
Catalog Number :
65828
Product Name :
Pflp-18::NGL-1::spGFP11
article :
doi10.1016/j.neuron.2007.11.030
id3150
pubmed_id18255029
bacterial resistance :
Ampicillin
cloning :
backbonepSM
backbone_mutation
backbone_originC.I. Bargmann and S. MaCarroll
backbone_size3500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
growth notes :
Mutation N142S does not affect plasmid function
growth strain :
For trans-synaptic labeling
origin :
37
pi :
alt_names
NLG-1 cDNA
cloning
clone_methodRestriction Enzyme
cloning_site_3PmeI
cloning_site_5NheI
promoterPflp-18
sequencing_primer_3
sequencing_primer_5GCCAAAGGACCCAAAGGTATG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_C40C9.5
genenlg-1
id181484
genbank_ids
mutationN142S
nameNLG-1
shRNA_sequence
size2610
species
6239
Caenorhabditis elegans
tags
locationN terminal on insert
tagspGFP11
plasmid copy :
Miri K. VanHoven made it originally, named as MVC12
resistance markers :
676
2510
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA