This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHis-zfCSF3b
catalog :
65613
citations: 2
Reference
Svoboda O, Stachura D, Machonova O, Zon L, Traver D, Bartunek P. Ex vivo tools for the clonal analysis of zebrafish hematopoiesis. Nat Protoc. 2016;11:1007-20 pubmed publisher
Stachura D, Svoboda O, Campbell C, Espin Palazon R, Lau R, Zon L, et al. The zebrafish granulocyte colony-stimulating factors (Gcsfs): 2 paralogous cytokines and their roles in hematopoietic development and maintenance. Blood. 2013;122:3918-28 pubmed publisher
product information
Catalog Number :
65613
Product Name :
pHis-zfCSF3b
article :
doi10.1182/blood-2012-12-475392
id10250
pubmed_id24128862
bacterial resistance :
Ampicillin
cloning :
backbonepETH2a
backbone_mutation
backbone_origin
backbone_size4500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
express soluble His-tagged zebrafish CSF3b/Gcsfb in E. coli
growth temp :
The depositing laboratory recommends strain BL21(DE3) for protein expression.
origin :
37
pi :
alt_names
Gcsf3b
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoterT7
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGcs, Gcsf, csf3
genecsf3b
id100190920
genbank_ids
NM_001143754
mutation
nameCsf3b
shRNA_sequence
size471
species
7955
Danio rerio
tags
locationN terminal on backbone
tag(His)6
resistance markers :
2399
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA