This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTre-Tight-luciferase-GFP (467)
catalog :
65491
citations: 1
Reference
Chen K, Chen J, Wang L, Yang J, Xiao F, Wang X, et al. Parkin ubiquitinates GATA4 and attenuates the GATA4/GAS1 signaling and detrimental effects on diabetic nephropathy. FASEB J. 2020;: pubmed publisher
product information
Catalog Number :
65491
Product Name :
pTre-Tight-luciferase-GFP (467)
article :
doi
id10237
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepTre-tight-SV40-luciferase
backbone_mutation
backbone_originGuigo lab, Addgene plasmid 65489
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth notes :
Note: Compared to the provided full plasmid sequence, Addgene's quality control sequencing finds an L27F amino acid residue substitution in the EGFP fluorophore. The depositing laboratory is aware of this residue change, and it does not affect fluorophore function.
growth strain :
negative control for expression assays
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promotertight TRE promoter
sequencing_primer_3C328R TATTACCGCCTTTGAGTGAGCTGA
sequencing_primer_5C328F AGGCGTATCACGAGGCCCTTTCGT
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameEGFP
shRNA_sequence
size734
species
tags
resistance markers :
2367
2686
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA