This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FUW-ubiquitin-EphB3-SV40-GFP (B3G)
catalog :
65443
citations: 3
Reference
Zhao K, He J, Wang Y, Jin S, Fan Y, Fang N, et al. EZH2-mediated epigenetic suppression of EphB3 inhibits gastric cancer proliferation and metastasis by affecting E-cadherin and vimentin expression. Gene. 2019;686:118-124 pubmed publisher
Xu T, Wang W, Ma P, Shuai Y, Zhao K, Wang Y, et al. Upregulation of the long noncoding RNA FOXD2-AS1 promotes carcinogenesis by epigenetically silencing EphB3 through EZH2 and LSD1, and predicts poor prognosis in gastric cancer. Oncogene. 2018;37:5020-5036 pubmed publisher
Cortina C, Palomo Ponce S, Iglesias M, Fernández Masip J, Vivancos A, Whissell G, et al. EphB-ephrin-B interactions suppress colorectal cancer progression by compartmentalizing tumor cells. Nat Genet. 2007;39:1376-83 pubmed
product information
Catalog Number :
65443
Product Name :
FUW-ubiquitin-EphB3-SV40-GFP (B3G)
article :
doi10.1038/ng.2007.11
id10225
pubmed_id17906625
bacterial resistance :
Ampicillin
cloning :
backboneFUW
backbone_mutation
backbone_originAddgene Plasmid 14882
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
PacI-AscI will excise the entire ubiquitin promoter-EphB3-SV40-GFP cassette. BamHI-AgeI will excise EPHB3.
growth strain :
Expression of EPHB3 and GFP in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3AscI
cloning_site_5PacI
promoterUBQ
sequencing_primer_3EGFP-N
sequencing_primer_5hUBCpro-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesEK2, ETK2, HEK2, TYRO6
geneEPHB3
id2049
genbank_ids
mutation
nameEPHB3
shRNA_sequence
size3002
species
9606
Homo sapiens
tags
locationC terminal on insert
tagSV40-EGFP
resistance markers :
2364
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA