This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pOPINB-OTUB1*
catalog :
65441
citations: 2
Reference
Michel M, Komander D, Elliott P. Enzymatic Assembly of Ubiquitin Chains. Methods Mol Biol. 2018;1844:73-84 pubmed publisher
Michel M, Elliott P, Swatek K, Simíček M, Pruneda J, Wagstaff J, et al. Assembly and specific recognition of k29- and k33-linked polyubiquitin. Mol Cell. 2015;58:95-109 pubmed publisher
product information
Catalog Number :
65441
Product Name :
pOPINB-OTUB1*
article :
doi10.1016/j.molcel.2015.01.042
id10223
pubmed_id25752577
bacterial resistance :
Kanamycin
cloning :
backbonepOPINB
backbone_mutation
backbone_originOPPF-UK
backbone_size5531
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses activated OTUB1 in E. coli
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7 terminator
sequencing_primer_5T7 promoter
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesE2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH4, UBCH5B
geneUBE2D2
id7322
genbank_ids
mutationActive site cysteine mutated to alanine
nameUbcH5b
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis-3C
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3T7 terminator
sequencing_primer_5T7 promoter
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHSPC263, OTB1, OTU1
geneOTUB1
id55611
genbank_ids
mutation
nameotubain-1
shRNA_sequence
size
species
tags
resistance markers :
2189
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA