This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLPCX-Cx43-FS-IRES-GFP
catalog :
65438
citations: 1
Reference
Salat Canela C, Sesé M, Peula C, Ramon Y Cajal S, Aasen T. Internal translation of the connexin 43 transcript. Cell Commun Signal. 2014;12:31 pubmed publisher
product information
Catalog Number :
65438
Product Name :
pLPCX-Cx43-FS-IRES-GFP
article :
doi10.1186/1478-811X-12-31
id10222
pubmed_id24884945
bacterial resistance :
Ampicillin
cloning :
backbonepLPCX
backbone_mutation
backbone_originClontech
backbone_size6264
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
FrameShift Mutation at EcoNI site of Cx43 (35delA) leading to out of frame synthesis after 11 amino acid and premature stop at amino acid 100.
growth strain :
Mammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral production
origin :
37
pi :
alt_names
Connexin 43
GJA1
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3LNCX-3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA
geneGJA1
id2697
genbank_ids
2697
mutationframeshift (FS) mutation 35delA
nameCx43
shRNA_sequence
size2575
species
9606
Homo sapiens
tags
locationC terminal on insert
tagIRES-GFP
plasmid copy :
Initially cloned into pBluescript: Cx43 insert digested out with EcoRV from Addgene plasmid #27383 and IRES-GFP digested from pIRES2-EGFP Addgene plasmid 3178 from clontech using BamHI-NotI. Entire Cx43-IRES-GFP cloned from pBluescript into pLPCX using XhoI and NotI.
resistance markers :
2361
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA