This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLPCX-Cx43-IRES-GFP
catalog :
65433
citations: 5
Reference
Werner R, Nekritz E, Yan K, Ju B, Shaner B, Easton J, et al. Single-cell analysis reveals the Comma-1D cell line as a unique model for mammary gland development and breast cancer. J Cell Sci. 2022;135: pubmed publisher
Li B, Wei Y, Li Q, Chen N, Li J, Liu L, et al. Nanomechanical Induction of Autophagy-Related Fluorescence in Single Cells with Atomic Force Microscopy. Adv Sci (Weinh). 2021;8:e2102989 pubmed publisher
Tishchenko A, Azorín D, Vidal Brime L, Muñoz M, Arenas P, Pearce C, et al. Cx43 and Associated Cell Signaling Pathways Regulate Tunneling Nanotubes in Breast Cancer Cells. Cancers (Basel). 2020;12: pubmed publisher
Kim S, Kwon H, Im S, Son Y, Akindehin S, Jung Y, et al. Connexin 43 is required for the maintenance of mitochondrial integrity in brown adipose tissue. Sci Rep. 2017;7:7159 pubmed publisher
Salat Canela C, Sesé M, Peula C, Ramon Y Cajal S, Aasen T. Internal translation of the connexin 43 transcript. Cell Commun Signal. 2014;12:31 pubmed publisher
product information
Catalog Number :
65433
Product Name :
pLPCX-Cx43-IRES-GFP
article :
doi10.1186/1478-811X-12-31
id10222
pubmed_id24884945
bacterial resistance :
Ampicillin
cloning :
backbonepLPCX
backbone_mutation
backbone_originClontech
backbone_size6264
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth strain :
Mammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral production
origin :
37
pi :
alt_names
Connexin 43
GJA1
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3LNCX-3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA
geneGJA1
id2697
genbank_ids
2697
mutation
nameCx43
shRNA_sequence
size2576
species
9606
Homo sapiens
tags
locationC terminal on insert
tagIRES-GFP
plasmid copy :
Initially cloned into pBluescript: Cx43 insert digested out with EcoRV from Addgene plasmid #27383 and IRES-GFP digested from pIRES2-EGFP Addgene plasmid 3178 from clontech using BamHI-NotI. Entire Cx43-IRES-GFP cloned from pBluescript into pLPCX using XhoI and NotI.
resistance markers :
2361
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA