Str-STIM1-NN_puromycin | Addgene 65310 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Str-STIM1-NN_puromycin

catalog :

65310

citations: 1

Reference
Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latrèche L, et al. Synchronization of secretory protein traffic in populations of cells. Nat Methods. 2012;9:493-8 pubmed publisher

product information

Catalog Number :

65310

Product Name :

Str-STIM1-NN_puromycin

article :

doi	10.1038/nmeth.1928
id	10202
pubmed_id	22406856

bacterial resistance :

Ampicillin

cloning :

backbone

pIRESpuro3

backbone_mutation

backbone_origin

Clontech

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

luminal tagging

growth strain :

Luminal ER hook only (RUSH system)

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	synth-int-R
sequencing_primer_5	CMV Forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	D11S4896E, GOK, IMD10, STRMK, TAM, TAM1
gene	STIM1
id	6786

genbank_ids

mutation

2 amino acids of STIM1 (IP from SxIP motif) were mutated to NN to abolish microtubule binding

name

STIM1 mutated for binding motif to microtubule

shRNA_sequence

size

species

9606

Homo sapiens