This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Str-KDEL_ST-SBP-EGFP
catalog :
65264
citations: 5
Reference
Pakdel M, Pacheco Fernandez N, von Blume J. Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Live-cell Vesicle Tracking in the Secretory Pathway Using HeLa Cells. Bio Protoc. 2021;11:e3958 pubmed publisher
Pacheco Fernandez N, Pakdel M, von Blume J. Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Protein Vesicle Tracking in HeLa Cells. Bio Protoc. 2021;11:e3936 pubmed publisher
Hecht T, Blank B, Steger M, Lopez V, Beck G, Ramazanov B, et al. Fam20C regulates protein secretion by Cab45 phosphorylation. J Cell Biol. 2020;219: pubmed publisher
Deng Y, Pakdel M, Blank B, Sundberg E, Burd C, von Blume J. Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network. Dev Cell. 2018;47:464-478.e8 pubmed publisher
Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latrèche L, et al. Synchronization of secretory protein traffic in populations of cells. Nat Methods. 2012;9:493-8 pubmed publisher
product information
Catalog Number :
65264
Product Name :
Str-KDEL_ST-SBP-EGFP
article :
doi10.1038/nmeth.1928
id10202
pubmed_id22406856
bacterial resistance :
Ampicillin
cloning :
backbonepIRESneo3
backbone_mutation
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
luminal tagging
growth strain :
synchronize trafficking of ST from the ER (RUSH system)
origin :
37
pi :
alt_names
ST6GAL1
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3EGFP-N
sequencing_primer_5IRES-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDw75, SIAT1, ST6GalI, ST6N
geneST6GAL1
id6480
genbank_ids
mutationcontains only amino acids 1 to 43 of ST
nameStr-KDEL and truncated sialyltransferase
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagStreptavidin Binding Protein (SBP)
locationC terminal on insert
tagEGFP
resistance markers :
2350
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA