This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-PTK7DN-VSV
catalog :
65251
citations: 1
Reference
Gartner S, Gunesch A, Knyazeva T, Wolf P, Högel B, Eiermann W, et al. PTK 7 is a transforming gene and prognostic marker for breast cancer and nodal metastasis involvement. PLoS ONE. 2014;9:e84472 pubmed publisher
product information
Catalog Number :
65251
Product Name :
pcDNA3-PTK7DN-VSV
article :
doiPONE-D-13-22093 [pii]
id10199
pubmed_id24409301
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutationin-frame VSV tag between ApaI and XhoI sites of pcDNA3
backbone_originInvitrogen
backbone_size5446
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Insert codes for extracellular and transmembrane domains of PTK7.
growth strain :
Expression of VSV tagged dominant negative PTK7 in mammalian cells
origin :
37
pi :
alt_names
CCK4DN
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3TGGCAACTAGAAGGCACAG
sequencing_primer_5CACTGCTTACTGGCTTATCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCCK-4, CCK4
genePTK7
id5754
genbank_ids
NM_002821
mutationdeleted AA 737-1071
namePTK7DN
shRNA_sequence
size2282
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagVSV
resistance markers :
2353
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA