This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-HA-ERK5kin
catalog :
65245
citations: 1
Reference
Buschbeck M, Eickhoff J, Sommer M, Ullrich A. Phosphotyrosine-specific phosphatase PTP-SL regulates the ERK5 signaling pathway. J Biol Chem. 2002;277:29503-9 pubmed
product information
Catalog Number :
65245
Product Name :
pcDNA3-HA-ERK5kin
article :
doi10.1074/jbc.M202149200
id10197
pubmed_id12042304
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5446
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Insert codes for kinase domain of ERK5 (AA 2-409)
growth strain :
Expression of HA tagged ERK5 kinase domain in mammalian cells
origin :
37
pi :
alt_names
MAPK7kin
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3TGGCAACTAGAAGGCACAG
sequencing_primer_5CACTGCTTACTGGCTTATCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBMK1, ERK4, ERK5, PRKM7
geneMAPK7
id5598
genbank_ids
NM_139033
mutationdeleted AA 410-817
nameERK5kin
shRNA_sequence
size1200
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
resistance markers :
2353
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA