This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRK5RS-HERCD533
catalog :
65224
citations: 1
Reference
Redemann N, Holzmann B, von Ruden T, Wagner E, Schlessinger J, Ullrich A. Anti-oncogenic activity of signalling-defective epidermal growth factor receptor mutants. Mol Cell Biol. 1992;12:491-8 pubmed
product information
Catalog Number :
65224
Product Name :
pRK5RS-HERCD533
article :
doi
id10189
pubmed_id1346334
bacterial resistance :
Ampicillin
cloning :
backbonepRK5
backbone_mutationmodified MCS
backbone_originBD PharMingen
backbone_size4754
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Insert codes for AA 1-653 (extracellular and transmembrane domains) Note: Amino acid number may be off when compared to current NCBI entry. Please check QC sequence to verify end of coding region.
growth strain :
Expression of dominant negative EGFR in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5XbaI
promoterCMV
sequencing_primer_3TATAAGCTGCAATAAAC
sequencing_primer_5CATACGATTTAGGTGACACTATAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA
geneEGFR
id1956
genbank_ids
NM_005228
mutationdeleted AA 654-1186
nameHERCD533
shRNA_sequence
size3900
species
9606
Homo sapiens
tags
resistance markers :
2353
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA