This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-Delta(Pro-MP)ADAM17-HA
catalog :
65221
citations: 1
Reference
Gschwind A, Hart S, Fischer O, Ullrich A. TACE cleavage of proamphiregulin regulates GPCR-induced proliferation and motility of cancer cells. EMBO J. 2003;22:2411-21 pubmed
product information
Catalog Number :
65221
Product Name :
pcDNA3-Delta(Pro-MP)ADAM17-HA
article :
doi10.1093/emboj/cdg231
id10174
pubmed_id12743035
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutationin-frame HA tag between ApaI and XhoI sites of pcDNA3
backbone_originInvitrogen
backbone_size5446
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Mutagenesis creates EcoRV site 57bp downstream of Start ATG. Insert contains several point mutations compared to NM_003183 (see sequence).
growth strain :
Expression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3TGGCAACTAGAAGGCACAG
sequencing_primer_5CACTGCTTACTGGCTTATCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesADAM18, CD156B, CSVP, NISBD, NISBD1, TACE
geneADAM17
id6868
genbank_ids
NM_003183
mutationdeleted AA 18-473
nameDelta(Pro-MP)ADAM17
shRNA_sequence
size1100
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagHA
resistance markers :
2353
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA