This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMSCV-flag-myr-Akt1-IRES-GFP
catalog :
65063
citations: 3
Reference
Kanasugi J, Hanamura I, Ota A, Karnan S, Lam V, Mizuno S, et al. Biallelic loss of FAM46C triggers tumor growth with concomitant activation of Akt signaling in multiple myeloma cells. Cancer Sci. 2020;111:1663-1675 pubmed publisher
Kurkewich J, Hansen J, Klopfenstein N, Zhang H, Wood C, Boucher A, et al. The miR-23a~27a~24-2 microRNA cluster buffers transcription and signaling pathways during hematopoiesis. PLoS Genet. 2017;13:e1006887 pubmed publisher
Kharas M, Okabe R, Ganis J, Gozo M, Khandan T, Paktinat M, et al. Constitutively active AKT depletes hematopoietic stem cells and induces leukemia in mice. Blood. 2010;115:1406-15 pubmed publisher
product information
Catalog Number :
65063
Product Name :
pMSCV-flag-myr-Akt1-IRES-GFP
article :
doi10.1182/blood-2009-06-229443
id10155
pubmed_id20008787
bacterial resistance :
Ampicillin
cloning :
backbonepMSCV-IRES-GFP
backbone_mutation
backbone_origin
backbone_size6500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
the plasmid is the property of Dana-Farber Cancer Institute
growth strain :
mammalian retroviral expression plasmid
origin :
37
pi :
alt_names
pkb
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR1
cloning_site_5EcoR1
promoter
sequencing_primer_3MSCV LTR 3'
sequencing_primer_5MSCV LTR 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
geneAKT1
id207
genbank_ids
mutation
nameAkt1
shRNA_sequence
size1661
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmyristoylation tag
locationN terminal on insert
tagflag
plasmid copy :
insert cDNA was from Jean Zhao at Dana-Farber Cancer Institute
resistance markers :
2238
613
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA