This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pWPXL-resNP1
catalog :
65061
citations: 1
Reference
Figueiro Silva J, Gruart A, Clayton K, Podlesniy P, Abad M, Gasull X, et al. Neuronal pentraxin 1 negatively regulates excitatory synapse density and synaptic plasticity. J Neurosci. 2015;35:5504-21 pubmed publisher
product information
Catalog Number :
65061
Product Name :
pWPXL-resNP1
article :
doi10.1523/JNEUROSCI.2548-14.2015
id10153
pubmed_id25855168
bacterial resistance :
Ampicillin
cloning :
backbonepWPXL
backbone_mutationEGFP removed and replaced with insert.
backbone_originDidier Trono
backbone_size10510
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
The mutations introduce a new EcoR1 restriction site on bp 459 of CDS to allow distinction between wildtype and resistant NP1. However, this prevents excision of the whole resNP1 insert from the pWPXL vector using EcoR1 restriction site
growth strain :
Constitutive expression of NP1 resistant to silencing by RNAi with pLVTH-shNP1
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3PmeI
cloning_site_5PmeI
promoterEF1alpha
sequencing_primer_3
sequencing_primer_5EF1a-F
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliases
geneNptx1
id266777
genbank_ids
NM_153735.2
mutationG447A, C450T, A451T, G452C, C454A, C456A, C459G
nameNeuronal Pentraxin 1
shRNA_sequence
size1302
species
10116
Rattus norvegicus
tags
resistance markers :
2335
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA