This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMS-eGFP-RTP801
catalog :
65057
citations: 4
Reference
Nogami A, Okada K, Ishida S, Akiyama H, Umezawa Y, Miura O. Inhibition of the STAT5/Pim Kinase Axis Enhances Cytotoxic Effects of Proteasome Inhibitors on FLT3-ITD-Positive AML Cells by Cooperatively Inhibiting the mTORC1/4EBP1/S6K/Mcl-1 Pathway. Transl Oncol. 2019;12:336-349 pubmed publisher
Zhang Z, Chu S, Wang S, Jiang Y, Gao Y, Yang P, et al. RTP801 is a critical factor in the neurodegeneration process of A53T α-synuclein in a mouse model of Parkinson's disease under chronic restraint stress. Br J Pharmacol. 2018;175:590-605 pubmed publisher
Barakat D, Mendonca J, Barberi T, Zhang J, Kachhap S, Paz Priel I, et al. C/EBPβ regulates sensitivity to bortezomib in prostate cancer cells by inducing REDD1 and autophagosome-lysosome fusion. Cancer Lett. 2016;375:152-161 pubmed publisher
Malagelada C, Ryu E, Biswas S, Jackson Lewis V, Greene L. RTP801 is elevated in Parkinson brain substantia nigral neurons and mediates death in cellular models of Parkinson's disease by a mechanism involving mammalian target of rapamycin inactivation. J Neurosci. 2006;26:9996-10005 pubmed
product information
Catalog Number :
65057
Product Name :
pCMS-eGFP-RTP801
article :
doi10.1523/JNEUROSCI.3292-06.2006
id10151
pubmed_id17005863
bacterial resistance :
Ampicillin
cloning :
backbonepCMS-EGFP
backbone_mutation
backbone_originClontech
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
There are 2 mutations if compared to PubMed DDIT4 R. Norvegicus sequence (L121V and S208G) because the insert was cloned out of DC12 cells DNA.
growth strain :
Expression of RTP801 in mammalian cells
origin :
37
pi :
alt_names
RTP801
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5EcoRI
promoterCMV-IE
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRtp801
geneDdit4
id140942
genbank_ids
140942
mutation
nameDDIT4
shRNA_sequence
size690
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagEGFP
resistance markers :
2351
2337
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA