This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRRL-MHCK7-GCaMP6
catalog :
65042
citations: 6
Reference
Dietz J, Jacobsen F, Zhuge H, Daya N, Bigot A, Zhang W, et al. Muscle Specific Promotors for Gene Therapy - A Comparative Study in Proliferating and Differentiated Cells. J Neuromuscul Dis. 2023;10:575-592 pubmed publisher
Massih B, Veh A, Schenke M, Mungwa S, Seeger B, Selvaraj B, et al. A 3D cell culture system for bioengineering human neuromuscular junctions to model ALS. Front Cell Dev Biol. 2023;11:996952 pubmed publisher
Ebrahimi M, Lad H, Fusto A, Tiper Y, Datye A, Nguyen C, et al. De novo revertant fiber formation and therapy testing in a 3D culture model of Duchenne muscular dystrophy skeletal muscle☆. Acta Biomater. 2021;: pubmed publisher
Afshar M, Abraha H, Bakooshli M, Davoudi S, Thavandiran N, Tung K, et al. A 96-well culture platform enables longitudinal analyses of engineered human skeletal muscle microtissue strength. Sci Rep. 2020;10:6918 pubmed publisher
Shadrin I, Allen B, Qian Y, Jackman C, Carlson A, Juhas M, et al. Cardiopatch platform enables maturation and scale-up of human pluripotent stem cell-derived engineered heart tissues. Nat Commun. 2017;8:1825 pubmed publisher
Madden L, Juhas M, Kraus W, Truskey G, Bursac N. Bioengineered human myobundles mimic clinical responses of skeletal muscle to drugs. elife. 2015;4:e04885 pubmed publisher
product information
Catalog Number :
65042
Product Name :
pRRL-MHCK7-GCaMP6
article :
doi10.7554/eLife.04885
id10141
pubmed_id25575180
bacterial resistance :
Ampicillin
cloning :
backbonepRRL
backbone_mutation
backbone_origin
backbone_size6904
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterMHCK7
sequencing_primer_3ttttgtaatccagaggttga
sequencing_primer_5gcctcgaccggtcgatctcg
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameGCaMP6
shRNA_sequence
size1353
species
100
Synthetic
tags
locationN terminal on insert
tag6xHis
locationN terminal on insert
tagT7 tag
locationN terminal on insert
tagXpress epitope
resistance markers :
2338
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA