This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-Lyso-TORCAR
catalog :
64929
citations: 2
Reference
Iffland P, Everett M, Cobb Pitstick K, Bowser L, Barnes A, Babus J, et al. NPRL3 loss alters neuronal morphology, mTOR localization, cortical lamination, and seizure threshold. Brain. 2022;: pubmed publisher
Zhou X, Clister T, Lowry P, Seldin M, Wong G, Zhang J. Dynamic Visualization of mTORC1 Activity in Living Cells. Cell Rep. 2015;10:1767-1777 pubmed publisher
product information
Catalog Number :
64929
Product Name :
pcDNA3-Lyso-TORCAR
article :
doi10.1016/j.celrep.2015.02.031
id10122
pubmed_id25772363
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5446
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3BGH reverse
sequencing_primer_5T7 forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD107a, LAMPA, LGP120
geneLAMP1
id3916
genbank_ids
mutation
nameLAMP1-TORCAR
shRNA_sequence
size3078
species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on insert
tagCerulean
locationC terminal on insert
tagYPet
locationN terminal on insert
tagFull-length LAMP1
resistance markers :
2182
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA