This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-TORCAR
catalog :
64927
citations: 9
Reference
Chen Z, He Q, Lu T, Wu J, Shi G, He L, et al. mcPGK1-dependent mitochondrial import of PGK1 promotes metabolic reprogramming and self-renewal of liver TICs. Nat Commun. 2023;14:1121 pubmed publisher
Yang J, Chi W, Liang J, Takayanagi S, Iglesias P, Huang C. Deciphering cell signaling networks with massively multiplexed biosensor barcoding. Cell. 2021;184:6193-6206.e14 pubmed publisher
Gopallawa I, Kuek L, Adappa N, Palmer J, Lee R. Small-molecule Akt-activation in airway cells induces NO production and reduces IL-8 transcription through Nrf-2. Respir Res. 2021;22:267 pubmed publisher
Fedder Semmes K, Appel B. The Akt-mTOR Pathway Drives Myelin Sheath Growth by Regulating Cap-Dependent Translation. J Neurosci. 2021;41:8532-8544 pubmed publisher
Fang C, Xie H, Zhao J, Wang W, Hou H, Zhang B, et al. eIF4E-eIF4G complex inhibition synergistically enhances the effect of sorafenib in hepatocellular carcinoma. Anticancer Drugs. 2021;32:822-828 pubmed publisher
Rabanal Ruiz Y, Byron A, Wirth A, Madsen R, Sedlackova L, Hewitt G, et al. mTORC1 activity is supported by spatial association with focal adhesions. J Cell Biol. 2021;220: pubmed publisher
Yamanaka R, Shindo Y, Hotta K, Suzuki K, Oka K. GABA-Induced Intracellular Mg2+ Mobilization Integrates and Coordinates Cellular Information Processing for the Maturation of Neural Networks. Curr Biol. 2018;: pubmed publisher
Zhou X, Li S, Zhang J. Tracking the Activity of mTORC1 in Living Cells Using Genetically Encoded FRET-based Biosensor TORCAR. Curr Protoc Chem Biol. 2016;8:225-233 pubmed publisher
Zhou X, Clister T, Lowry P, Seldin M, Wong G, Zhang J. Dynamic Visualization of mTORC1 Activity in Living Cells. Cell Rep. 2015;10:1767-1777 pubmed publisher
product information
Catalog Number :
64927
Product Name :
pcDNA3-TORCAR
article :
doi10.1016/j.celrep.2015.02.031
id10122
pubmed_id25772363
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5446
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
FRET-based biosensor for monitoring mTORC1 kinase activity.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3BGH reverse
sequencing_primer_5T7 forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliases4E-BP1, 4EBP1, BP-1, PHAS-I
geneEIF4EBP1
id1978
genbank_ids
mutation
nameTORCAR
shRNA_sequence
size1803
species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on insert
tagCerulean
locationC terminal on insert
tagYPet
resistance markers :
2182
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA