This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRSET-XS-VWF
catalog :
64847
citations: 2
Reference
Gogia S, Lo C, Neelamegham S. Detection of Plasma Protease Activity Using Microsphere-Cytometry Assays with E. coli Derived Substrates: VWF Proteolysis by ADAMTS13. PLoS ONE. 2015;10:e0126556 pubmed publisher
Dayananda K, Gogia S, Neelamegham S. Escherichia coli-derived von Willebrand factor-A2 domain fluorescence/Förster resonance energy transfer proteins that quantify ADAMTS13 activity. Anal Biochem. 2011;410:206-13 pubmed publisher
product information
Catalog Number :
64847
Product Name :
pRSET-XS-VWF
article :
doi10.1016/j.ab.2010.12.005
id9876
pubmed_id21146487
bacterial resistance :
Ampicillin
cloning :
backbonepRSET
backbone_mutation
backbone_origin
backbone_size2807
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses aa1594 1670 of VWF A2 domain flanked by Venus and Cerulean and tagged with 7X His at C terminus
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7 Terminator
sequencing_primer_5T7 promoter
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesF8VWF, VWD
geneVWF
id7450
genbank_ids
NM_000552.3
DQ092360.1
mutation
nameVenus_VWF A2 domain aa 1594-1670_Cerulean_TEV_7X His
shRNA_sequence
size1713
species
9606
Homo sapiens
100
Synthetic
tags
locationC terminal on insert
tag7X His
locationN terminal on insert
tagVenus
locationC terminal on insert
tagCerulean
resistance markers :
2323
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA